In Vitro Study of Radiation-induced Dna Damage
نویسندگان
چکیده
This work presents the effects of low-LET radiations (electrons) on normal and modified cells DNA. The radiation-induced effects are assessed using the COMETassay. The results show that modified cells are more sensible to exposure than the normal ones. The dose-effect relationship follows a linear-no-threshold model suggested by BEIR VII.
منابع مشابه
Antioxidant capacity and radioprotective properties of the flavonoids galangin and kaempferide isolated from Alpinia galanga L. (Zingiberaceae) against radiation induced cellular DNA damage
Background: Alpinia galanga L. belonging to the family Zingiberaceae is widely grown in the state of Kerala, India. They are effective antioxidant and free radical scavenger under both in vitro and in vivo condition. The efficacy of the isolated flavonoids in conferring protection from radiation induced damages to genomic DNA was studied. Materials and Methods: The bioflavonoids, galangin and k...
متن کاملIn-vitro evaluation of rutin and rutin hydrate as potential radiation countermeasure agents
Background: DNA damage is one of the major consequences of radiation exposure onto the biological systems. A series of compounds including flavanoids were found to render DNA protection against radiation damage. In this study we elucidated the potential of rutin and rutin hydrate to protect plasmid DNA against damage induced by irradiation. Materials and Methods: DPPH and hydroxyl radical scave...
متن کاملStudies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay
Background: Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of cellular water. Since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the DNA damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملEvaluation of DNA damage in a Her2+ cell line induced by an Auger-emitting immunoconjugate
Introduction: Auger electron based radioimmunotherapy (RIT) using 111In-DOTA-trastuzumab (111In-DOTA-antiHer2) feasibility was studied in vitro on a HER2/neu positive cell line, SkBr3. Methods:111In-DOTA-antiHer2 was prepared according to the optimized conditions followed by quality control tests including radioch...
متن کاملConsumption of antioxidant dietary agents, curcumin and vitamin C, protects cellular DNA from gamma-radiation
Background: Exposure to ionizing radiation results in genotoxicity and the unrepaired lesions in cellular DNA results in cell cycle arrest, reproductive death, interphase death, division delay, chromosome aberrations, mutations, etc. leading to the intensive destruction of cells and violation of their proliferative capacity there by adversely affecting the mammalian system. Since ionizing radia...
متن کاملI-37: Genome Instability and DNA Damage in Male Somatic and Germ Cells Expressed as Chromosomal Microdeletion and Aneuploidy Is A Major Cause of Male Infertility
Background: Sperm chromatin insufficiencies leading to low sperm count and quality, infertility and transmission of chromosomal microdeletion and aneuploidies to next generations can be due to exposure to environmental pollutions, chemicals and natural or manmade ionizing radiation. In this project which has continued for more than 10 years and is unique in many technical aspects in Iran and in...
متن کامل